Herald of Technological University Herald of Technological University ВЕСТНИК ТЕХНОЛОГИЧЕСКОГО УНИВЕРСИТЕТА 3034-4689 62904 БИОХИМИЯ, БИОТЕХНОЛОГИЯ И ЭКОЛОГИЯ БИОХИМИЯ, БИОТЕХНОЛОГИЯ И ЭКОЛОГИЯ THE INFLUENCE OF THE TREATMENT BY THE CLEANED SOLUBLE PROTEINS TO THE FRAGMENTED SARCOPLASMIC RETICULUM Alekseeva O M Alekseeva O M olgavek@yandex.ru Zaikov G E Zaikov G E Emanuel Institute of Biochemical Physics, Russian Academy of Sciences ru Emanuel Institute of Biochemical Physics, Russian Academy of Sciences ru KNRTU ru KNRTU ru 01 08 2025 01 08 2025 17 7 173 178 20 04 2023 https://vestniktu.ru/en/nauka/article/62904/view

Основной целью работы было подтверждение существенной роли свободных жирных кислот в проявлении пассивной проницаемости мембран саркоплазматического ретикулума, являющегося основным кальциевым депо в скелетных мышцах млекопитающих. Для решения этой задачи была использована способность молекул альбумина адсорбировать и освобождать свободные жирные кислоты и другие вещества при изменениях окружающей среды. Альбумин сыворотки крови человека (САЧ) в виде водных растворов полностью освобождали от адсорбированных гидрофобных веществ с помощью активированного фармацевтического древесного угля при низких значениях рН. После удаления угля центрифугированием возвращали рН раствора альбумина к нейтральному - физиологическому. Молекулы альбумина принимали нативную глобулярную форму, полностью освобожденную от адсорбированных веществ. Инкубация с «чистым» САЧ фрагментированного саркоплазматического ретикулума значительно снижала пассивную проницаемость мембран для ионов кальция.

The main goal of this work was the supporting of the essential role of free fatty acids to the passive permeability of the basic muscles calcium store - sarcoplasmic reticulum, for the calcium ions. The probability of albumin molecules to absorb and release the free fatty acids and another absorbed substances under the certain variable environments was used for this problems decide. In order to solve this task the aqueous solution of human serum albumin (HSA) was freed from adsorbed hydrophobic ligands fully. The albumin molecules were treated by medium with low pH in presence of pharmaceutical activated charcoal. After deposition of coal by means of centrifugation, the pH of medium was changed back on physiology. In this case the albumin molecule bought its native globular form, but already has been free from all adsorbed substances, which were moved away together with charcoal. Further the preparations of the fragmented sarcoplasmic reticulum were treated with such clean albumin. The passive sarcoplasmic reticulum permeability for calcium ions decreased considerably. So, that the essential role of free fatty acids for the efficiency of basic calcium store of muscles - sarcoplasmic reticulum for the calcium ions was confirmed. And, than the albumin’s absorption ability was confirmed by the cleaning of sarcoplasmic reticulum preparations from free fatty acids.

Human serum albumin sarcoplasmic reticulum Сывороточный альбумин человека саркоплазматический ретикулум пассивная проницаемость passive permeability Human serum albumin sarcoplasmic reticulum Сывороточный альбумин человека саркоплазматический ретикулум пассивная проницаемость passive permeability

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