The bacterial capability to rapidly adapt to unfavorable environment conditions is determined by biosynthesis of variety proteins. Under nitrogen limitation, in Bacilli the transcription factor TnrA controls the genes of nitrogen metabolism. Under nitrogen excess conditions its activity is repressed by the feedback inhibited form of glutamine synthetase. Here we report the negative regulation of transcription factor TnrA by glutamine synthetase even under conditions of nitrogen limitation. The activity of TnrA in the cells is repressed under conditions despite of high activity of glutamine synthetase. At that in the glutamine synthetase deficient cells the TnrA-dependent transcription was 7-times higher in compare with a wild type cells. We attribute this increasing to the higher amount of TnrA in the mutant strain.
фактор транскрипции TnrA, глутаминсинтетаза, регуляции активности, transcription factor TnrA, glutamine synthetase, regulation of activity
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