This work describes the preparation of the sludge from JSC «Kazanorgsintez» for bioremediation and optimization of sludge treatment parameters. Field experiment was conducted to prepare the sludge for bioremediation by means of natural freezing-thawing and agrotechnical methods. Achieved changes in sludge’s structure and subsequent reduction of its phytotoxicity allow us to carry out phytoremediation free of mixing the sludge with bulking agents. Forms and doses of fertilizers were optimized with respect to the best respiratory activity of microorganisms. Tolerant forms of plants performing phytoremediation were screened. Our data suggest that sludge containing hydrocarbons might be remediated more effectively after natural freezing-thawing and agrotechnical treatment.
шлам, замораживание и оттаивание, подготовка шлама к биологическому обезвреживанию, биоремедиация, фиторемедиация, sludge, freeze-thaw, preparation for bioremediation, bioremediation, phytoremediation
1. Van Hamme J.D., Singh A., Ward O.P., Microbiol. Mol. Biol. Rev., 67, 4, 503-549 (2003)
2. D.J. Jean, C.P. Chu, D. Lee, Separ. Sci. Technol., 36, 12, 2733-2746 (2001)
3. C. Lin, G. He, X. Li, L. Peng, C. Dong, S. Gu, G. Xiao, Separ. Purif. Technol., 56, 2, 175-183 (2007)
4. R.L. Rhykerd, B. Crews, K.J. Mcinnes, R.W. Weaver, Bioresour. Technol., 67, 279-285 (1999)
5. G. Chen, G. He, Separ. Purif. Technol., 31, 1, 83-89 (2003)
6. A.A. Shinkarev, K.G. Giniyatullin, L.V. Mel'nikov, G.A. Krinari, S.G. Gnevashev, Organicheskie komponenty glino-metallo- organicheskogo kompleksa pochv lesostepi (teoreticheskie i eksperimental'nye aspekty izucheniya), Izd-vo KGU., Kazan', 2007. 249 c.
7. A. A. Nesmelov, T. V. Grigor'eva, R. H. Huzayanov, A. A. Smolko, I. R. Muhametshin, E.V. Nikitina, E. V Gicareva., R. P. Naumova, Vestn. Kaz. tehnol. Un-ta., 14, 177-181 (2012)
8. F. Schinner, E. Kandeles, R. Őhlinger, R. Margesin, Methods in Soil Biology, Springer, Berlin, 1995. 426 p.
9. ISO 11269-1 «Soil quality - determination of the effects of pollutants on soil flora - Part 1: Method for measurement of inhibition of root growth», International Standard Organisation, Geneva, 1993. 13p.
10. R. Juvonen, E. Martikainen, E. Schultz, A. Joutti, J. Ahtiainen, M.A. Lehtokari, Ecotox. Environ. Safe., 47, 156-166 (2000)
11. M.K Banks, H. Mallede, K. Rathbone, Soil Sediment Contam., 3, 371-385 (2003).
12. W. April, R.C. Sims, Chemosphere, 20, 253-265 (1990)
13. D. E. Salt, R. D. Smith, I. Raskin, Annu. Rev. Plant Physiol. Plant Mol. Biol., 49, 643-668 (1998)
14. J.L. Kirk, J.N. Klironomos, H. Lee, J.T. Trevors, Environ. Pollut., 133. - P.455-465 (2005).
15. H.I. Atagana, R.J. Haynes, F.M. Walli, Biodegradation, 14, 4, 297-307 (2003)
16. W. Ouyang, H. Liu, V. Murygina, Y. Yu, Z. Xiu, S. Kalyuzhnyi, Process Biochem, 40, 3763-3768 (2005)
