In this paper we present the comprehensive method for isolation of bacterial viruses from different ecological niches of Almetyevsky District (from the Almetyevsk city and the rural area Boriskino) and Vysokogorsky District (from the rural area Kurkachi) in the Republic of Tatarstan (Russia). Soils from forest and field (Boriskino), humus (Kurkachi and Boriskino), urban oil-polluted and non-polluted soils (Almetyevsk city), as well as rural pond water (Boriskino) served as sources of bacterial viruses. Seven gram-positive and gram-negative bacteria were used as indicator strains. We obtained collection composed of 26 bacterial viruses specifically targeting 7 indicator strains. Most of viruses were discovered in bacteria related to genus Bacillus.
вирусы бактерий, бактериофаги, почва, природная вода, метод выделения бактериофагов, коллекция бактериофагов, bacterial viruses, bacteriophages, soil, natural water, method for bacteriophage isolation, bacteriophage collection
1. Abedon, S. T. Bacteriophage prehistory / S. T. Abedon ,C. Thomas-Abedon, A. Thomas and H. Mazure // Bacteriophage. - 2011. - V. - 1, No. 3. - P. 174-178.
2. d’Herelle, F. Sur un microbe invisible antagoniste des bacillus Dysentériques / F. d’Herelle // Comptes rendus Acad. Sci. Paris. - 1917. - V.165. - P.373-375.
3. Brüggemann H. Bacteriophages Infecting Propionibacterium acnes / H. Brüggemann, R. Lood // Biomed. Res. Int. - 2013. - P. 1-10. (doi.org/10.1155/2013/705741)
4. Sulakvelidze, A. Bacteriophage Therapy / A. Sulakvelidze, Z. Alavidze, J.G. Jr.Morris // Antimicrob. Agents Chemother. - 2001. - V. 45. - I. 3. - P. 649-659.
5. Lu, T.K. The next generation of bacteriophage therapy / T.K. Lu, M.S. Koeris // Curr. Opin. Microbiol. - 2011. - V. 14. - I. 5. - P. 524-531.
6. Levin, B. R. Phage therapy revisited: the population biology of a bacterial infection and its treatment with bacteriophage and antibiotics / B. R. Levin, J. J. Bull // Am. Nat. 1996. - V. 147. - I. 6. - P. 881-898.
7. Barrow, P.A. Bacteriophage therapy and prophylaxis: rediscovery and renewed assessment of potential / P.A. Barrow , J.S. Soothill // Trends Microbiol. - 1997. - V. 5. - No. 7. - P. 268-271.
8. Bar, H. Killing cancer cells by targeted drug-carrying phage nanomedicines / H. Bar, I. Yacoby, I. Benhar // BMC Biotechnology. - 2008. - V. 8. - No. 37. - P. 1- 14. (doihttps://doi.org/10.1186/1472-6750-8-37)
9. Baggesen, D.L. Phage typing of salmonella typhimurium - is it still a useful tool for surveillance and outbreak investigation? / D.L. Baggesen, G. Sorensen1, E. M. Nielsen, H. C. Wegener // Eurosurveillance. -2010. - V. 15. I. 4. (pii=19471)
10. Kropinski A.M. Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay / A. M. Kropinski, A. Mazzocco, T. E. Waddell, E. Lingohr, R. P. Johnson //Methods in Mol. Biol. - 2009. - V. 501. - P. 69-76.
11. Guntupalli, R. Detection and identification of methicillin resistant and sensitive strains of Staphylococcus aureus using tandem measurements / R. Guntupalli, I. Sorokulova, E. Olsen, L. Globa, O. Pustovyy, T. Moore, B. Chin, J. Barbaree, V. Vodyanoy // J. Microbiol. Methods. - 2012. - V. 90. - No. 3. - P. 182-191.
12. García, P. Bacteriophages and their application in food safety / P. García , B. Martínez , J.M. Obeso, A. Rodríguez // Lett. Appl. Microbiol. - 2008. - V. 47. - I. 6 P. 479-885 (doi:https://doi.org/10.1111/j.1472-765X.2008.02458.x)
13. Abedon, S.T. Phage treatment of human infections / S.T. Abedon, S.J. Kuhl, B.G. Blasdel, E.M. Kutter // Bacteriophage. - 2011. - V.1. - I. 2. - P. 66-85. (doihttps://doi.org/10.4161/bact.1.2.15845)
14. Whitman, W. B. Prokaryotes: the unseen majority / W. B. Whitman, D.C. Coleman, W.J. Wiebe // Proc. Nat. Acad. Sci. USA. - 1998. - V. 95. - I. 12. - P. 6578-6583.
15. Leung, E. The WHO policy package to combat antimicrobial resistance / E. Leung, D.E. Weil, M. Raviglione, H. Nakatani // Bull. World Health Organ. - 1211. - V.89. - P. 390-392. (doihttps://doi.org/10.2471/BLT.11.088435)
