The three genera of Enterobacteriaceae bacteria - Proteus, Morganella, Providencia were identified by microbiological and molecular-biological methods. Extracellular and intracellular proteolytic activities of these strains were investigated with the use of various protein substrates. Proteus mirabilis has high extracellular proteolytic activity. Intracellular proteases cleaving gelatine were found in Morganella and Providencia cell extracts.
идентификация, энтеробактерии, протеиназы, зимография, identification, enterobacteriaceae, proteinases, zymography
1. C.M. O'Hara, F.W. Brenner, J.M. Miller, Clin Microbiol Rev., 13, 4, 534-546 (2000).
2. D. Juyal, V.K. Rathaur, N. Sharma, J. Clin Diagn Res., 7, 2, 369-370 (2013).
3. K. Tanaka, T. Haraguchi, F. Yamamichi, M. Muramaki, H. Miyake, M. Fujisawa, Korean J Urol., 54, 3, 189-193 (2013).
4. M.M. Pearson, M. Sebaihia, C. Churcher, M. A. Quail, A.S. Seshasayee, N.M. Luscombe, J Bacteriol., 190, 4027-4037 (2008).
5. P. Van, R. Belas, B.F. Gilmore, H. Ceri1, Infection and Immunity, 76, 11, 4859-4864 (2008).
6. N.V. Belonogova, A.B. Margulis, V.Ya. Ponamarev, A.I. Kolpakov, O.N. Il'inskaya, Vestnik Kazanskogo tehnologicheskogo universiteta, 15, 23, 109-112 (2012).
7. J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, NY, 1989, 2344 p.
8. M.M. Bradford, Anal. Biochem., 72, 248-254 (1976).
9. O.K. Pozdeev, Medicinskaya mikrobiologiya. GEOTAR-MED, Moskva, 2001, 765 s.
10. J.M. Janda, S.L. Abbott, J. Clin. Microbiol., 45, 9, 2761-2764 (2007).
11. C. Wassif, D. Cheek, R. Belas, J. Bacteriol., 177, 20, 5790-5798 (1995).
12. O.A. Caplina, T.N. Efremova, L.V. Kever, Ya.Yu. Komissarchik, I.V. Demidyuk, S.V. Kostrov, S.Yu. Haytlina, Biohimiya, 74, 797-804 (2009).
13. U.K. Laemmli, Nature (London), 227, 680-685 (1970).
14. G.W. Oliver, W.G. Stettler-Stevenson, D.E. Kleiner, In: Handbook of proteolyitic enzymes. Acad. Press, San Diego, 1999, P. 61-76.
15. B. W. Senior, J. Med. Microbiol., 48, 623-628 (1999).
