The transcription factors TnrA and GlnR, members of the MerR family of transcription regulators, control the genes of the nitrogen metabolism in Bacillus subtilis under conditions of nitrogen deficiency and excess, respectively. Their DNA-binding activity is regulated by the interaction with the glutamine synthetase, the key enzyme of ammonium assimilation. By surface plasmon resonance it has been established that glutamine synthetase has a different effect on the activity of these transcription factors. In the presence of inhibited glutamine synthetase, the DNA-binding activity of TnrA is decreased, while GlnR affinity to DNA increases. This fact correlates with the activity of TnrA and GlnR in various conditions of nitrogen availability and characterizes the glutamine synthetase as a trigger enzyme that controls nitrogen metabolism.
Bacillus subtilis, факторы транскрипции TnrA, GlnR, глутаминсинтетаза, Bacillus subtilis, transcription factors TnrA and GlnR, glutamine synthetase
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