In this study, we characterized 5’-regulatory regions of protease genes from B. pumilus 3-19. To define the minimal size of protease gene promoters, we generated and analyzed expression of gfp fusion-reporter constructs harboring full-length and truncated promoters of different lengths. To find regulators which orchestrate the expression of serine proteases, activity of reporter constructs were further explored in recombinant B. subtilis mutants defective in regulatory genes. Our results indicate positive regulation of expression for all protease genes by DegU and Spo0A proteins but this regulation is different.
промотор, протеиназы, экспрессия, репортерный ген, факторы транскрипции, Bacillus pumilus, promoter, proteases, gene expression, reporter gene, transcription factors, Bacillus pumilus
1. J.W. Veening, O.A. Igoshin, R.T. Eijlander, R. Nijland, L.W. Hamoen, O.P. Kuipers, Mol Syst Biol., 4, 184 (2008).
2. A.A. Toymenceva, A.I. Ahmetova, M.R. Karimova, Ch. Nyamsuren, Yu.O. Ponomareva, E.I. Shagimardanova, A.A. Rizvanov, M.R. Sharipova, Mikrobiologiya, 82, 1, 59 (2013).
3. L. Westers, H. Westers, G. Zanen, H. Antelmann, M. Hecker, D. Noone, K.M. Devine, J.M. van Dijl, W.J. Quax, Proteomics, 8, 13, 2704-2713 (2008).
4. D. López, R. Kolter, FEMS Microbiol Rev., 34, 2, 134-149 (2010).
5. N.M. Zamalyutdinova, I.R. Galieva, A.O. Arapova, E.O. Mihaylova, M.R. Sharipova, A.M. Mardanova, Vestnik Kazanskogo tehnologicheskogo universiteta, 16, 191-195 (2013).
6. F.A. Davidson, C. Seon-Yi, N.R. Stanley-Wall, PLoS One, 7, 6, e38574 (2012).
7. M.K. Dahl, T. Msadek, F. Kunst, G. Rapoport, J. Biol. Chem., 267, 20, 14509-11454 (1992).
8. S.M. Ruzal, C. Sanchez-Rivas, Curr. Microbiol., 37, 368-372 (1998).
9. F. Kunst, G. Rapoport, J. Bacteriol., 177, 2403-2407 (1995).
10. M. Fujita, J.E. Gonzalez-Pastor, R. Losick, J. Bacteriol., 187, 1357-1368 (2005).
11. V. Molle, M. Fujita, S.T. Jensen, P. Eichenberger, J.E. Gonzalez-Pastor, Mol. Microbiol., 50, 1683-1701 (2003).
12. A. Sánchez, J. Olmos Bacillus subtilis transcriptional regulators interaction, Biotechnol Lett., 26, 5, 403-407 (2004).
13. K. Tsukahara, M. Ogura, FEMS Microbiol. Lett., 280, 1, 8-13 (2008).
14. S. Abe, A. Yasumura, T. Tanaka, J. Bacteriol., 191, 9, 3050-3058 (2009).
15. C. Borgmeier, R. Biedendieck, K. Hoffmann, D. Jahn, F. Meinhardt, Microbiol. Biotechnol., 92, 3, 583-596 (2011).
16. P. Bisicchia, E. Botella, K.M., Plasmid, 64, 143-149 (2010).
17. H.C. Birnboim, J. Doly, Nucleic Acids Res, 7, 1513-1523 (1979).
18. C.R. Harwood, S.M. Cutting Molecular Biological Methods for Bacillus, Chichester: Wiley, England, 1990,581 p.
19. E. Botella, M. Fogg, M. Jules, S. Piersma, G. Doherty, A. Hansen, E.L. Denham, L. Le Chat, P. Veiga, K. Bailey, P.J. Lewis, J.M. van Dijl, S. Aymerich, A.J. Wilkinson, K.M. Devine, Microbiology, 156, 1600-1608 (2010).
20. M. Sharipova, N. Balaban, A. Kayumov, Y. Kirillova, A. Mardanova, L. Gabdrakhmanova, I. Leshchinskaya, G. Rudenskaya, T. Akimkina, D. Safina, I. Demidyuk, S. Kostrov, Microbiol Res., 163, 1, 39-50 (2008).
21. M.R. Sharipova, E.I. Shagimardanova, I.B. Chastukhina, T.R. Shamsutdinov, N.P. Balaban, A.M. Mardanova, G.N. Rudenskaya, I.V. Demidyuk, S.V. Kostrov, Mol. Biol. Rep., 34, 2, 79-87 (2007).
22. A.R. Sabirova. Diss. kand. biol. nauk, K(P)FU, Kazan', 2011. 150 s.
