Using bioinformatic analysis of Morganella morganii subsp. genome we identified gene homologous of grimelyzin gene of Serratia grimesii. We identified genes orthologous to the gene of M. morganii hypothetical metalloprotease in the genomes of several species of Enterobacteriaceae and representatives of other families. In the promoter region of metalloprotease M. morganii -10 and -35 sites and also Shine-Dalgarno sequence were identified. Using primers designed for M. morganii subsp. KT gene metalloprotease gene from M. morganii ZM clinical isolate was amplified. PCR-amplificate sequencing and BLAST processing revealed 83% gomology of the genes.
Morganella morganii, протеиназа, биоинформационный анализ, промотор, Morganella morganii, proteinase, bioinformatic analysis, promoter
1. P. Zalas-Wiecek, E. Gospodarek, J. Wróblewska, Przegl. Epidemiol., 66, 1, 13-18 (2012).
2. C. Zhao, N. Tang, Y. Wu, Y. Zhang, Z. Wu, W. Li, X. Qin, J. Zhao, G. Zhang, Vet. Microbiol., 156, 452-455 (2012).
3. J. Abdalla, M. Saad, I. Samnani, P. Lee, J. Moorman, Am. J. Med. Sci., 331, 1, 44-47 (2006).
4. H. Atalay, I. Güney, Y. Solak, E. Almaz, Perit. Dial. Int., 30, 1, 119-121 (2010).
5. N.M. Zamalyutdinova, I.R. Galieva, A.O. Arapova, E.O. Mihaylova, M.R. Sharipova, A.M. Mardanova, Vestnik Kazanskogo tehnologicheskogo universiteta, 16, 10, 191-194 (2013).
6. A.M. Mardanova, E.H. Nizamutdinova, T.V. Shirshikova, L.H. Kamaletdinova, E.O. Mihaylova, M.R. Sharipova, L.M. Bogomol'naya, Vestnik Kazanskogo tehnologicheskogo universiteta, 19, 215-218 (2013).
7. Y.T. Chen, H.L. Peng, W.C. Shia, F.R. Hsu, C.F. Ken, Y.M.Tsao, C.H. Chen, C.E. Liu, M.F. Hsieh, H.C. Chen, C.Y. Tang, T.H. Ku, BMC Genomics, 13, (2012).
8. E.S. Bozhokina, S.Yu. Khaitlina, T. Adam, Biochem. Biophys. Res. Commun., 367, 888-892 (2008).
9. E.S. Bozhokina, O.A. Tsaplina, T.N. Efremova, L.V. Kever, I.V. Demidyuk, S.V. Kostrov, T. Adam, Y.Y. Komissarchik, S.Y. Khaitlina, Cell. Biol. Int., 35, 2, 111-118 (2011).
10. J. Sambrook, E.F. Fritsch, S.T. Maniatis, Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, NY, 1989, 2344 p.
