Three different methods based on quantification of the liberated phosphates, as well as quantification of the decrease in phytate concentration during the enzymatic reaction were compared. Quantification ofthe liberated phosphates with ammonium-molybdate method was found to be the best way todetermine total phytate-degrading activity of P. vagans 3.2 phytase in crude extract.
фитаза, фитазная активность, инозитолфосфаты, phytase, phytase assay, inositolphosphates
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