The main goal of this work was the supporting of the essential role of free fatty acids to the passive permeability of the basic muscles calcium store - sarcoplasmic reticulum, for the calcium ions. The probability of albumin molecules to absorb and release the free fatty acids and another absorbed substances under the certain variable environments was used for this problems decide. In order to solve this task the aqueous solution of human serum albumin (HSA) was freed from adsorbed hydrophobic ligands fully. The albumin molecules were treated by medium with low pH in presence of pharmaceutical activated charcoal. After deposition of coal by means of centrifugation, the pH of medium was changed back on physiology. In this case the albumin molecule bought its native globular form, but already has been free from all adsorbed substances, which were moved away together with charcoal. Further the preparations of the fragmented sarcoplasmic reticulum were treated with such clean albumin. The passive sarcoplasmic reticulum permeability for calcium ions decreased considerably. So, that the essential role of free fatty acids for the efficiency of basic calcium store of muscles - sarcoplasmic reticulum for the calcium ions was confirmed. And, than the albumin’s absorption ability was confirmed by the cleaning of sarcoplasmic reticulum preparations from free fatty acids.
Human serum albumin, sarcoplasmic reticulum, Сывороточный альбумин человека, саркоплазматический ретикулум, пассивная проницаемость, passive permeability
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