The main task of this investigation was the clearing of some aspects of Melafen influence on the structural properties of membrane bounded proteins and lipids. Melafen (melamine salt of bis (oximethyl) phosphinic acid) is applied in agriculture, as strong plant growth regulator. Melafen was used as aqua solutions, at wide concentration’s region at all our investigations. The isolated from blood erythrocytes, erythrocyte’s ghost were used as the experimental objects. The Melafen interactions with membrane bounded proteins at the erythrocyte’s ghost were tested by the differential scanning microcalorymetry (DSC) method. The application of DSC allowed us to receive the structural data of protein microdomains organization at erythrocyte’s ghost membranes. We did not reveal any noticeable structural changes of the proteins of erythrocyte ghost membranes under the concentrations of Melafen, when concentrations of Melafen were so much bigger, than concentrations of Melafen, which are used at crop production. The Melafen actions to the protein-lipid interrelationships were tested by registrations of the microviscosity of isolated native erythrocytes. The using of electron spin resonance method (ESR) with introduction of spin-labelled probes to erythrocytes allowed us to receive some structural data of protein -lipid organization at the different depths of erythrocyte’s membranes (2-4 Å; 4-8 Å). The applauding of spectral method for registration of hemolysis degree, allowed us to receive some data about Melafen influences to membrane resistance under the bed environment, when the hypo- and hyper-osmolarity existed. We concluded that Melafen has not any destruction’s influences to the membranes bounded proteins under the concentrations that activate the plant growth. But Melafen had essential influencing to the structural and functional properties of membrane bounded proteins when the bigger concentrations.
Melafen, erythrocyte, erythrocyte ghost, differential microcalorymetry, microviscosity, hemolysis, мелафен, эритроциты, тени эритроцитов, дифференциальная сканирующая микрокалориметрия, микровязкость, гемолиз
1. Fattachov S.G., Reznik V.S., Konovalov A.I. “Melamine Salt of Bis (hydroxymethyl) phosphinic Acid. (Melaphene) As a New Generation Regulator of Plant growth regulator” // Set of articles. Reports of 13 International Conference on chemistry of phosphorus compounds. S. Petersburg. 2002. S. 80.2.
2. Zhigacheva I.V., Fatkulina L.D., Burlakova E.B., Shugaev A.I., Generosova I.P., Fattahov S.G., Konovalov A.I. “ Influence of phosphoorganic plant growth regulator to the structural characteristics of membranes plant’s and animal’s origin” // Biological membrane. 2008. V. 25. № 2. P. 150-156.
3. Zhigacheva I.V., Misharina T.A., Trenina M.B., Krikunova N.N., Burlakova E.B., Generosova I.P., Shugaev A. I., Fattahov S.G. “Fatty acid’s content of mitochondrial membranes of Pea seedlings in conditions of insufficient moistening and treatment by the phosphoorganic plant growth regulator” // Biological membrane. 2010. V. 27. P. 256-261.
4. Alekseeva O.M., Krivandin A.V., Shatalova O.V., Rykov V.A., Fattakhov S.G., Burlakova E.B., Konovalov A.I. “The Melafen-Lipid- interaction Determination in phospholipid membranes” // “Reports of Russian Academy of Sciences” (in Rus.). 2009. V. 427. N. 6. P. 218-220.
5. Dodge.J.T., Mitchell C., Hanahan D. J. “The preparation and chemical characteristics of hemoglobin-free ghost of human erythrocytes” // Arch. Biochem. Biophys. 1963. V. 100. P. 199-130.
6. Sato Y., Yamakose H. Suzuki Ya. “Mechanism of hypotonic hemolysis of human erythrocytes” // Biol. Pharm. Bull. 1993. V. 16. № 5. P. 506-512.
7. Privalov P.L., Plotnikov V.V. “Three generations of scanning microcalorimeters for liquids” // Therm. Acta. 1989. V.139. P. 257-277.
8. Jackson W.M., Kostyla J., Nordin J.H., Brandts J.F. “Calorimetric study of protein transitions in human erythrocyte ghosts” // Biochemistry. 1973. V.12. P. 3662-3667.
9. Brandts J.F., Lysko K.A., Schwartz A.T., Eryckson L., Carlson R.B., Vincentelli J., Taverna R.D. “Structural studies of the erythrocyte membrane” // Colloques. Internationaux. du. C. R. S. 1976. V. 246. P. 169-175.
10. Mohandas N., Greenquis, A.C., Shohet S.B. “Effect of heat and metabolic depletion on erythrocyte deformability, spectrin extractability and phosphorilation” // The Red Cell. New York. Alan R. Liss. Inc. 1978. P. 453-472.
11. Heath B.P., Mohandas N., Wyatt J.L., Shohet S.B. “Deformability of isolated red cells” // Biochim.et biophis. Acta. 1982. V. 691. № 2. P. 211-219
12. Akoev V.R., Sherbinina S.P., Matveev A.V., Tarachovsky Yu. S., Deev A.A., Shnirov V.L. “Investigations of structural transitions at erithricytes membrane when hereditary hemochromatosis” // Bulletin Experimental biology and medicine 1997. V. 123. № 3. P. 279-284.
13. Jackson W.M., Kostyla J., Nordin J.H., Brandts J.F. “Calorimetric study of protein transitions in human erythrocyte ghosts” // Biochemistry. 1973. V.12. № 19. P. 3662-3667.
14. Gulevski A.K., Riazancev V.V., Belous A.M. “Structural transitions of lipids and proteins of erythrocytes membrane when low temperature action” // Scientific Reports of High School. Biol. Sciences. (in Russ.) 1990. P. 29-36.
15. Akoev V.R., Matveev A.V., Belyaeva T.V., Kim Y.A.” "The effect of oxidative stress on structural transitions of human erythrocyte ghost membranes"// Biochim Biophys Acta. 1998. V. 1371. №2. P. 284-294.
16. Goloshchapov A.N., Burlakova E.B. “Microviscosity definition and structural transitions into lipids and proteins of cell membranes by the method of spin probes” // Biophysics. 1975. V. 20. № 5. P. 816-821.
17. Jager F.C. “Determination of vitamin E requirement in rats by means of spontaneous haemolysis in vitro” // Nutr. et Diets. 1968. V. 10. № 3. P.215−223.
18. Shnyrov V.L., Zhadan G.G., Salia C.H. “Thermal transitions in rat erythrocyte ghosts” // Biomed. Biochim. Acta. 1986. V. 45. P. 1119-1121.
19. Binukov V.I., Borunova S.F., Goldfeld M.G. “Paramagnetic probes at EPR-spectroscopic” // Biochemistry. 1972. V. 36. № 6. P. 1149-1153.
20. Vasserman A.M., Kovarsky A.L. “Spin probes and zondes at physicochemistry and polymers” M. Science (Nauka) 1986. 255 p.
21. Zhigacheva I.V., Fatkulina L.D., Burlakova E.B., Shugaev A.I., Fattahov S.G., Konovalov A.I “Preparation Melafen and physical-chemical state of membane ”/ Reports of Russian Academy of Sciences” (in Rus.). 2006. V. 409. № 4. P. 547-549
22. Kim Yu.A., Elemesov R.E., Akoev V.R. “Hyper osmotic hemolysis of erythrocytes and antihemolytic activity of saponins faction and triterpens glycosides from Panax Ginseng C.A.Meyer” // Biological Membrane. 2000. V.17. № 2. P. 15-26.
