Alterations of molecular parameters of glycinin, soybean storage 11S globulin, generated by papain limited proteolysis have been described. The decrease of molecular mass, hydrodynamic radius, radius of gyration, surface charge and asymmetry of glycinin molecule as well as the increase of its surface hydrophobicity were shown using the methods of laser static and dynamic light scattering, small angle X-ray scattering and a high-speed sedimentation. In general, the described alterations of glycinin molecular parameters coincide with those of its subunit primary structure previously found occurring during limited proteolysis by papain.
глицинин, соевые бобы, ограниченный протеолиз, папаин, молекулярные параметры, светорассеяние, glycinin, soybeans, limited proteolysis, papain, molecular parameters, light scattering
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