It is shown by NMR relaxation methods, that during the centrifugation in case of intensive and(or) long enough impact of mechanical forces on the yeast preparation there take place not only the process of microbiological pellet concentration, but the saturation of intracellular yeasts space with a physiologically surplus quantity of а liquid (water) too, which leads to а partial cell walls damage and forthcoming yeast cells plasmolysis.
дрожжевой препарат, обезвоживание, центрифугирование, плазмолиз, ЯМР релаксометрия, структурно-динамическое состояние, yeast preparation, dewatering, centrifugation, plasmolysis, NMR relaxometry, structural-dynamical state
1. Glik, B. Molekulyarnaya biotehnologiya. Principy i primenenie./B. Glik, Dzh. Pasternak. - M.:Mir, 2002. -589 s.
2. Farrar, T. Impul'snaya i Fur'e-spektroskopiya YaMR: per. s angl. pod red. E.I. Fedina. / T. Farrar, E. Bekker. - M.: Mir, 1973.- 164 s.
3. Metody obschey bakteriologii. V treh tomah. T. 2. / Pod red. F. Gerhardta i dr.- M.: Mir, 1984. - 470 s.
4. Stejskal, E.D. Self-Diffusion Measurements: Spin Echoes in Presence of a Time Dependent Field Gradient / E.D. Stej-skal, J.E. Tanner // J. Chem. Phys., 1965.- V. 42.- № 1 - P.288 - 292.
5. Tanner, J.E. Restricted Self-Diffusion of Protons in Colloidal Systems by the Pulsed-Gradient Spin-Echo Method / J.E. Tanner, E.O. Stejskal // J. Chem. Phys., 1968. - V. 49. - P. 1768.
6. Resing, H.A. Apparent Phase-Transition Effect in the NMR Spin-Spin Relaxation Time Caused by a Distribution of Correlation Times. / H.A. Resing // J. Chem. Phys., 1965. - V. 43. - № 2. - P. 669.
7. Zimmerman, J.R. Nuclear Magnetic Resonance Studies in multiple phase Systems: lifetime of a water molecule in an absorbing phase on silicagel. / J.R. Zimmerman, W.E. Britten. // J. Phys. Chem., 1957. - V.61. - № 9 - P. 1328 - 1333.
8. Skirda, V.D. Vliyanie dinamiki mezhfaznogo obmena na YaMR-relaksaciyu./V.D. Skirda, A.V. Sevryugin, A.I. Mak-lakov // Him. Fizika, 1983. - №11. - S. 1499.
9. Vashman, A.A. Yadernaya magnitnaya relaksacionnaya spektroskopiya. / A.A. Vashman, I.S. Pronin // M.: Energoatomizdat, 1986. - 232 s.
10. Meiboom, S. Proton Relaxation in Water / S. Meiboom, Z. Luz, D. Gill // J. Chem. Phys., 1957.-V. 27 -№ 6.-P. 1411.
11. Migchelsen, C. Proton exchange and molecular orientation of water in hydrated collagen fibers. An NMR study of H2O and D2O / C. Migchelsen and H.J.C. Berendsen // J. Chem. Phys., 1973. - V. 59. - № 1. - P. 296.
12. Myunster, A. Himicheskaya termodinamika: per. s nem. k.h.n. E.P. Ageeva pod red. chl.-korr. AN SSSR Ya.I. Gerasimova / A. Myunster. - M.: Mir, 1971. - 295 s.
13. Kasatkin, A.G. Osnovnye processy i apparaty himicheskoy tehnologii / A.G. Kasatkin. - M.: Al'yans, 2005. - 753 s.
14. Romankov, P.G. Gidromehanicheskie processy himi-cheskoy tehnologii / P.G. Romankov, M.I. Kurochkina. - L.: Himiya, 1974. - 288 s.
15. Sibgatullin, T.A. Sovmestnyy analiz diffuzionnogo i relaksacionnogo povedeniya vody v kletkah myakoti yabloka./T.A. Sibgatullin, A.V. Anisimov, P.A. de Jager i dr.// Biofizika. - 2007. -T. 52. - № 2. - S. 268 - 276.
16. Sibgatullin, T.A. Translyacionnaya dinamika vody v citoplazme kletok parenhimy ploda Malus domestica. Issledovanie impul'snym metodom YaMR./T.A. Sibgatul-lin, F.Dzh. Vergel't, A.V. Anisimov, H. Van As. // Doklady RAN. - 2006. - T. 411. - № 3. - S. 427 - 429.
17. Komarevceva, I.A. Yaderno-magnitno-rezonansnaya relaksometriya protonov tkanevoy vody kozhi. / I.A. Ko-marevceva, E.N. Popov, A.V. Zaika, V.N. Komarevcev // Ukrainskiy zhurn. klinicheskoy i labor. mediciny, 2008. - T. 3. - № 1.
18. Krynickaya, A.Yu. Osobennosti individual'nogo i sovmestnogo deystviya kompleksona OEDF i KVCh - izlucheniya na rost hlebopekarnyh drozhzhey Saccharomyces cerevisiae. / A.Yu. Krynickaya, P.P. Suhanov, T.A.Opaleva i dr. // Vestnik Kazan. tehnol. un-ta.- 2004- № 1-2.- S.138 - 147.
19. Krynickaya, A.Yu. Vliyanie EMI KVCh na aktivnost' preparata «Fitosporin» / A.Yu. Krynickaya, P.P. Suha-nov, E.V. Petuhova i dr. // Vestnik Kazan. tehnol. un-ta.- 2011. - № 7. - S. 181-184.
20. Krynickaya, A.Yu. Izuchenie ksantana, poluchennogo pri kul'tivirovanii xanthomonas campestris na modificirovannyh sredah metodami IK-spektroskopii i YaMR-relaksometrii / A.Yu. Krynickaya, P.P. Suhanov, M.R. Hisametdinov i dr. // Vestnik Kazan. tehnol. un-ta.- 2009 - № 6. - S. 135 - 140.
